Exosome Transfection
Turning Exosomes into Delivery Vehicles
Harnessing exosomes as carriers of genetic material and small molecules opens new possibilities for cancer research, regenerative medicine, and drug discovery. A persistent challenge, however, has been how to efficiently and reproducibly load exosomes with functional cargo such as mRNA, siRNA, miRNA, or therapeutic compounds. Conventional methods like electroporation can cause aggregation and loss of vesicles, limiting downstream applications.
The Exo-Fect™ Exosome Transfection Kit provides a simple and scalable solution for exosome loading. This optimized reagent ensures high efficiency, minimal vesicle loss, and preserved exosome functionality for in vitro and in vivo experiments. By streamlining the workflow, Exo-Fect enables researchers to focus on results—whether studying biomarker transfer, therapeutic delivery, or basic exosome biology.
Key Benefits
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Efficient loading of nucleic acids (mRNA, miRNA, siRNA, DNA) and small molecules into exosomes
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Minimal aggregation and high recovery compared to electroporation or chemical alternatives
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Maintains exosome functionality for uptake and delivery studies
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Compatible with downstream analysis and in vivo applications
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Easy-to-use workflow that saves valuable research time
Applications
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Engineering exosomes as delivery shuttles for therapeutic RNA and DNA
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Studying intercellular communication via exosome cargo transfer
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Developing exosome-based drug delivery systems
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Enhancing biomarker discovery and functional assays
Frequently Asked Questions
Conventional methods like electroporation often cause aggregation and vesicle loss. The Exo-Fect™ kit provides a dedicated reagent system that efficiently incorporates RNA into purified exosomes while preserving their integrity, giving you reproducible results for downstream studies.
Yes. Exo-Fect is designed to transfect a wide variety of cargo, including plasmid DNA, oligos, proteins, and small molecules, while minimizing vesicle damage, making it highly versatile for therapeutic and functional applications.
That’s a key concern. With Exo-Fect, loaded exosomes retain their natural uptake and delivery properties, so you can confidently use them in both in vitro assays and in vivo models without losing functionality.
Yes. The Exo-Fect workflow is quick, consistent, and reproducible, making it practical for both small-scale tests and translational research requiring higher volumes.
In Germany, Exo-Fect is available exclusively through BioCat, ensuring you have local support and distribution. Learn more and order here »