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Exosome Isolation: SmartSEC Purification

System Bio’s SmartSEC technology addresses low-volume and high-throughput EV isolation needs with a single, reproducible approach.

SmartSEC Single

SmartSEC™ Single

SmartSEC™ Single
SmartSEC Mini

SmartSEC™ Mini EV Isolation System

SmartSEC™ Mini EV Isolation System
SmartSEC HT

SmartSEC™ HT EV Isolation System

SmartSEC™ HT EV Isolation System
SmartSEC-DeLipo 2

SmartSEC-DeLipo™

SmartSEC-DeLipo™

From Pin-Point Samples to 96-Well Power: SmartSEC™ Delivers Pure EVs at Any Scale

Unlock uncompromised extracellular vesicle isolation whether you’re working with a mere 10 µL of precious developmental-biology fluid or screening 96 clinical samples in under an hour.

SmartSEC combines a proprietary resin with contaminant-trapping chemistry and ready-to-use spin-column or 96-well formats to deliver faster, higher-purity, and more reproducible Exosome isolations. From single-drop inputs to high-throughput workflows, it consistently outperforms standard SEC’s slower, co-elution-prone protocols.

 

Why SmartSEC Outperforms Standard SEC

  • Proprietary Resin with Contaminant Trapping
    Unlike conventional SEC, which often co-elutes abundant plasma proteins and lipoproteins with EVs, SmartSEC incorporates a custom resin plus a “contaminant trapping” feature that selectively retains non-EV components—delivering cleaner fractions in a single step.
  • Optimized Formats for Every Scale
    Mini (Spin-column): Pre-loaded columns calibrated for as little as 10 µL of biofluid—no packing required—ideal for precious or low-volume samples.
    Single (Spin Column); Pre-loaded columns calibrated for as little as 100 µL of biofluid—no packing required—ideal for quick and easy exosome isolation.
    HT (96-well plate):     Pre-aliquoted resin in a filter plate lets you process 1–96 serum/plasma samples in under an hour with no plate prep or manual fraction collection.
  • Faster, Easier Workflows
    Load your sample, incubate (≈30 min for Mini; plate-wide for HT), then centrifuge twice to recover highly pure EVs—versus standard SEC’s multi-hour runs and fraction pooling.
  • Superior Purity, Yield & Reproducibility
    Gentle SEC separation plus contaminant trapping helps outperform ultracentrifugation and traditional SEC in both particle recovery and protein removal—ensuring reproducible results across labs and sample types.

Compatible Across Sample Types

  • Plasma/Serum: Clean, reproducible EV fractions suited for liquid biopsy and biomarker discovery.
  • Cell Culture Media: Consistent EV isolation for mechanistic studies and method development.

Go Even Further with SmartSEC-DeLipo™

Even with the best SEC methods, lipoproteins can remain a stubborn contaminant in plasma- and serum-derived EV preps. SmartSEC-DeLipo™ available as a standalone column or paired seamlessly with SmartSEC—selectively removes lipoproteins that co-elute with extracellular vesicles. The result: ultra-pure, lipoprotein-depleted EV fractions that maximize confidence in downstream RNA, proteomics, and biomarker analyses.

Key Specifications

Feature SmartSEC Single SmartSEC Mini SmartSEC HT
Reactions per kit 10 10 96
Sample volume 100 – 250 µl 10–100 µL 250–500 µL per well
Format Spin-columns Spin-columns 96-well filter plate
Incubation 30 min at RT 30 min at RT Plate-wide, simultaneous
Centrifugation 1 minute at 500 x g 30 s at 500 × g Standard plate centrifuge
Automation compatibility Manual Manual Manual or automated liquid handling
Downstream compatibility MS, NTA, TEM, WB, RNA-Seq MS, NTA, TEM, WB, RNA-Seq MS, NTA, TEM, WB, RNA-seq

Want to learn more? 

Access the SmartSECTM EV Isolation System technology introduction paper from System Bio.

Access the Application Note
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SmartSEC Single

  • Sample Volume: 100–250 µL of biofluid (serum, plasma, hemolymph, apoplastic fluid, culture media)

  • Format: Spin-column pre-loaded with SmartSEC resin, collection tubes, and buffer for 10 reactions

  • Performance:

    • Higher purity and yield than ultracentrifugation

    • Preserves EV integrity

  • Applications: Mass spectrometry, Western blotting, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), RNA-Seq

 

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smartsec-single-workflow

Frequently Asked SmartSEC Single Questions

What is the SmartSEC™ Single EV Isolation System?

The SmartSEC system is a spin-column kit that uses proprietary size-exclusion chromatography (SEC) resin to isolate extracellular vesicles (EVs) from as little as 100 µL of biofluid—making it ideal for quick and easy exosome isolation. It delivers higher purity and yield than ultracentrifugation in a rapid, user-friendly workflow.

Which biofluids and organisms has SmartSEC™ Single been validated for?

SmartSEC™ Single has been validated for human serum, plasma, and CSF as well as Aplysia californica hemolymph.

The SmartSEC™-TC Kit has been specifically designed and optimized for use with large volumes of tissue culture media, including stem cells.

How does SmartSEC™ Single compare to ultracentrifugation and other SEC methods?

Compared to ultracentrifugation, SmartSEC Single is faster, easier, and provides better purity and yield. It also provides higher EV concentration per fraction than conventional size exclusion chromatography kits

Which downstream applications are compatible with SmartSEC™ Single preps?

Isolated EVs are ready for mass spectrometry, RNA-seq, Western blot, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), RNA-Seq and other ‘omics or imaging workflows.

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SmartSEC Mini EV Isolation System

  • Sample Volume: 10–100 µL of biofluid (serum, plasma, hemolymph, apoplastic fluid, culture media)

  • Format: Spin-column pre-loaded with SmartSEC resin, collection tubes, and buffer for 10 reactions

  • Performance:

    • Higher purity and yield than ultracentrifugation

    • Preserves EV integrity

  • Applications: Mass spectrometry, Western blotting, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM)

 

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smartsec-mini-workflow-01-739x2400-1

Frequently Asked SmartSEC Mini Questions

What is the SmartSEC™ Mini EV Isolation System?

The Mini system is a spin-column kit that uses proprietary size-exclusion chromatography (SEC) resin to isolate extracellular vesicles (EVs) from as little as 10 µL of biofluid—making it ideal for precious clinical or developmental-biology samples. It delivers higher purity and yield than ultracentrifugation in a rapid, user-friendly workflow.

Which biofluids and organisms has SmartSEC™ Mini been validated for?

SmartSEC™ Mini has been validated on hemolymph from Drosophila melanogaster, culture media from Planaria, apoplastic fluid from Arabidopsis thaliana, and serum/plasma from human, mouse, and rat samples.

How does SmartSEC™ Mini compare to ultracentrifugation and other SEC methods?

Compared to ultracentrifugation, Mini is faster, easier, and requires 10- to 100-fold less sample volume while delivering superior purity and EV yield. It also outperforms competitor SEC columns in total EV recovery.

Which downstream applications are compatible with SmartSEC™ Mini preps?

Isolated EVs are ready for mass spectrometry, RNA-seq, Western blot, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and other ‘omics or imaging workflows.

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SmartSEC HT EV Isolation System for Serum & Plasma

  • Throughput: Up to 96 samples in a single 96-well plate run in < 1 hour 

  • Sample Volume per Well: 250–500 µL serum or plasma; partial-plate runs possible, unused wells remain sealed 

  • Features:

    • No pre-treatment of plasma required

    • Compatible with manual and automated liquid handlers

    • Purity/yield superior to ultracentrifugation and competitor SEC columns

  • Applications: EV biomarker discovery, diagnostics, therapeutics R&D

 

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SmartSEC-HT-Workflow-01-1

Frequently Asked SmartSEC HT Questions

What is the SmartSEC™ HT EV Isolation System?

SmartSEC™ HT is a 96-well plate–based SEC system designed for high-throughput EV isolation from serum or plasma. You can process up to 96 samples in under one hour with no plasma pre-treatment, all while maintaining high purity and yield.

What benefits does SmartSEC™ HT bring to a high-throughput EV workflow?

It accelerates biomarker discovery and therapeutic R&D by enabling rapid, simultaneous processing of dozens of samples, offering flexible partial-plate runs, and seamless compatibility with liquid-handling robots.

How does SmartSEC™ HT compare to ultracentrifugation?

SmartSEC™ HT consistently achieves higher EV purity and yield than ultracentrifugation—and even outperforms competitor SEC columns—in both protein and particle recovery assays.

What is the SmartSEC™ HT workflow?

  1. Prep the 96-well filter plate (with pre-aliquoted resin).

  2. Load 250–500 µL cleared serum or plasma per well.

  3. Incubate briefly, then centrifuge to elute Fraction 1.

  4. Add equal volume of Isolation Buffer and centrifuge again for Fraction 2.

  5. Analyze or pool fractions as needed.

Can I run partial plates and reuse unused wells?

Yes—unused wells stay sealed in the plate, allowing you to process subsets of samples now and return to remaining wells later.

Does SmartSEC™ HT handle both serum and plasma without pre-treatment?

Absolutely. After a brief spin to clear debris, load serum or plasma directly onto the plate—no additional prep steps required.

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Lipoprotein Removal Made Simple for Biomarker-Ready EVs

The SmartSEC-DeLipo™ kit takes extracellular vesicle isolation further than ever before. By uniting SmartSEC’s advanced mixed-mode chromatography with dedicated lipoprotein removal, it eliminates one of the biggest sources of noise in blood-derived EV preps. The result: cleaner fractions that reveal the true biological signals, free from lipoprotein-bound microRNAs and other confounders.

With a streamlined workflow, high yield, and compatibility across plasma, serum, breast milk, and more, SmartSEC-DeLipo delivers ultra-pure, lipoprotein-depleted sEVs ready for RNA, proteomics, and functional assays. Available in both column and 96-well HT formats, it ensures reproducibility from a single sample to entire cohorts—accelerating discovery and meeting the most demanding standards of rigor.

 


 

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SmartSEC-DeLipo

Frequently Asked SmartSEC-DeLipo Questions

What makes SmartSEC-DeLipo different from standard SEC or ultracentrifugation?

SmartSEC-DeLipo combines size-exclusion with affinity-based lipoprotein removal. Unlike traditional methods, it isolates EVs in a single fraction while trapping proteins and lipoproteins that can interfere with downstream analysis

Why is lipoprotein removal important in EV isolation from blood?

Lipoproteins carry their own RNA cargo, which can mask true exosomal signals in biomarker studies. Removing them ensures that downstream RNA, proteomics, or functional assays reflect only EV content, not confounding particles.

Which sample types are compatible with SmartSEC-DeLipo?

The kit is validated for plasma, serum, and breast milk, and is broadly compatible with other biofluids. This versatility allows researchers to confidently apply the workflow across multiple study designs

What formats are available for different scales of work?

SmartSEC-DeLipo is available in single-column and 96-well HT versions, enabling reproducible results from low-volume samples to large clinical cohorts

How easy is the workflow to implement?

The protocol is simple: load the sample, incubate, centrifuge, and pass the eluate through the DeLipo column. In less than an hour, researchers obtain ultra-pure, lipoprotein-depleted EVs ready for downstream assays.

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