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All you need for your Exosome research

Evidence‑based guidance for biogenesis, isolation, and analysis.

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Biogenesis

Biogenesis
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Isolation

Isolation
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Transfection

Transfection
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QC/Analysis

QC/Analysis

Exosome Biogenesis & Secretion

Exosomes originate in the endosomal system and are one of three main types of extracellular vesicles (EVs). 

Three main EVs, three origin stories:

  • Apoptotic bodies ≈ 0.5–5 µm — shed during apoptosis.

  • Microvesicles ≈ 150–1000 nm — bud from the plasma membrane.

  • Exosomes ≈ 30–150 nm — formed as ILVs inside multivesicular endosomes; released when microvesicles fuse with the membrane.
    (Size bands are approximate and vary by source.)

Cut noise before you start: use EV-depleted fetal bovine serum (FBS) and tight conditioning windows in culture.

 

Exosome Biogenesis
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Exosome Isolation

Isolation is a trade‑off between purity, yield, hands‑on time, and scalability.

  • Differential Ultracentrifugation (dUC): Flexible baseline where equipment/time are available. Expect invisible pellets; handle gently; confirm by NTA and markers.

  • Size Exclusion Chromatography (SEC): Gentle, plasma‑first for purity; works for serum and cell culture media. Plan fraction tracking and recovery math.

  • SmartSECTM Purification: SmartSEC combines a proprietary resin with contaminant‐trapping chemistry and ready‐to‐use spin-column or 96-well formats to deliver faster, higher-purity, and more reproducible EV isolations—from 10 µL samples to high-throughput workflows—outperforming standard SEC’s slower, co-elution-prone protocols

  • Polymer Precipitation (ExoQuick®): High‑throughput, fast. The ULTRA versions include a column clean‑up to reduce albumin/Ig carryover.

  • Silicon Carbide (SiC) Purification: Norgen’s Silicon Carbide (SiC) technology is a proprietary exosome purification method designed for a fast, simple isolation protocol with minimal contamination. 

Isolation Hub
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Transfection

Exosomes are nature’s own delivery vehicles—and with the right tools, they can be loaded with RNA, DNA, or small molecules for advanced research and therapeutic applications.

The Exo-Fect™ Exosome Transfection Kit, distributed in Germany by BioCat, offers a fast, reliable way to transfect exosomes without compromising vesicle integrity.

Transfection
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Exosome Analysis

High-performance profiling starts here.
Exosome research demands accuracy, speed, and workflows you can trust. From RNA profiling to proteomics, lipidomics, particle counting, and flow cytometry, BioCat brings together validated tools from leading innovators like Norgen Biotek and Systems Bio. Each kit and platform is designed to give you reproducible results, whether you’re chasing biomarker signatures in serum, plasma, or cell culture.

From isolation to insight.
With streamlined protocols, Exosome-specific labeling, and calibration standards built-in, these solutions remove the uncertainty from exosome analysis. The result: faster, cleaner data that can withstand reviewer scrutiny and help your lab publish with confidence. Whether you’re mapping exosomal RNA, quantifying vesicles, or uncovering protein and lipid signatures, this hub equips you to turn samples into discoveries.

Exosome Analysis Hub
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Frequently Asked Questions

What’s the most practical way to load mRNA into exosomes/EVs?

Two main routes: engineer donor cells (endogenous) or load purified EVs (exogenous). For speed and gentler handling, many labs start with an exosome-specific transfection reagent, see the Exo-Fect Exosome Transfection Kit

How do I make sure the mRNA is inside the EVs (not stuck outside)?

Run an RNase protection assay on aliquots ±detergent, then quantify by RT-qPCR/dPCR. Signal that survives RNase but disappears with RNase+detergent indicates internal cargo.

 

How do I reduce albumin/lipoproteins before proteomics?

For plasma/serum, run a size exclusion chromatography exosome isolation step (SEC) and keep EV‑rich, protein‑poor fractions. Avoid harsh depletion columns that bias cargo. If you used polymer exosome precipitation, either pick ExoQuick® ULTRA (built‑in clean‑up) or follow with an SEC polish to drop ApoA1/ApoB and albumin.

When should I use RNase pretreatment?

Use RNase (± detergent) when you need to distinguish external vs encapsulated RNA, especially in plasma studies or whenever reviewers will ask. Report exactly where you applied RNase (pre‑/post‑isolation) and include no‑RNase controls.

How do I know these kits won’t compromise my RNA or protein yield?

All workflows featured here are validated with leading EV isolation methods (ExoQuick, ultracentrifugation, SEC). They’re optimized to preserve vesicle integrity and maximize recovery, so you can trust the material you analyze truly reflects your EV population.

Will the data be reproducible enough for publication and grant reviewers?

Yes. Each system is designed with built-in controls—such as calibration standards for NTA or size markers for flow cytometry—that ensure consistency across runs. That means your data will withstand reviewer questions about reproducibility.

Can I use these tools across both discovery and translational projects?

Absolutely. Kits for RNA, protein, lipid, and particle analysis are optimized for biofluids, tissue culture, and low-volume clinical samples. They fit seamlessly into discovery experiments while supporting translational workflows where patient material is limited.

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