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Exosome Analysis

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High-Performance Exosome Profiling for Impactful Research

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RNA Analysis & Profiling

RNA Analysis & Profiling
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Protein Mass Spectrometry

Protein Mass Spectrometry
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Lipidomics

Lipidomics
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NTA Particle Counting

NTA Particle Counting
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Flow Cytometry

Flow Cytometry

RNA Analysis & Profiling

Advance your understanding of exosomal RNA with Norgen’s Exosome RNA Isolation Kit, engineered for maximal yield from plasma, serum, and cell culture supernatants, and Systems Bio’s SeraMir™ Exosome RNA Amplification System for downstream qPCR or NGS workflows.

Whether you’re troubleshooting low yield, selecting spike-in controls, or optimizing RNase-pretreatment, our RNA Analysis subpage guides you through best practices and normalization strategies to ensure reproducible profiling in miRNA- and lncRNA-focused studies.

 

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Protein Mass Spectrometry

Your complete workflow for protein-level insights.

The ExoMS™ line from System Biosciences makes extracellular vesicle protein analysis simple and reproducible. Whether you’re mapping the exosome from culture, serum, or plasma, ExoMS provides fast and clean capture for downstream discovery.

Why ExoMS?

  • Designed for compatibility with common EV isolation methods

  • Optimized for reproducibility across sample types

  • Streamlined workflows that save time and protect precious material

With ExoMS™, you can move seamlessly from vesicle prep to proteomic insight, turning your EV samples into meaningful biomarker data for discovery, validation, and translational research.

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Lipidomics

Unlock the lipidome of your extracellular vesicles with Systems Bio’s LipidEX™ Extraction Kit, designed for broad-spectrum recovery of sphingolipids and phospholipids, and Norgen’s Lipid Quantification Assay for high-sensitivity detection.

Our Lipidomics subpage covers protocol selection—Folch vs. Bligh & Dyer—plus MS-grade solvent recommendations, data normalization approaches, and tips to prevent lipid oxidation during storage, helping you map exosome membrane composition in cancer liquid biopsy studies.

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NTA Particle Counting

When particle counts matter for your grant reports and publications, you can’t afford background noise. Malvern’s NanoSight, paired with theExoGlow™-NTA Fluorescent Labelling Kit (the only commercially available EV-specific fluorescent labelling kit), gives you accurate size distribution and quantitation, without the uncertainty of non-vesicular contaminants.

  • See only EVs, not debris. Proprietary dye specifically labels extracellular vesicles, delivering high signal-to-noise you can trust.

  • Validated across workflows. Compatible with ExoQuick, ultracentrifugation, and SEC-based isolation.

  • Fast and reproducible. From isolated sample to analysis in just 30 minutes, optimized to keep your team moving.

This kit provides the confidence that your NTA readouts reflect vesicles, not artifacts.

Get the ExoGlow
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Flow Cytometry

Flow cytometry was never designed for extracellular vesicles—until now. With SBI’s ExoFlow-ONE Gemstone dyes, you can finally unlock the full power of flow-based methods for EV biology.

  • Direct detection. Specifically labels internal EV components for clear, reliable signals.

  • Sharper data. Built-in size standards ensure every run is properly calibrated.

  • More insight per sample. Multiplex with spectrally separated dyes to ask more questions from the same prep.

  • Near single-vesicle resolution. Proprietary high-efficiency dyes reveal details once hidden in the noise.

The result? Flow cytometry that goes beyond bulk measurements—delivering sharper, cleaner, and more meaningful insights into EV biology.

Go with the ExoFlow
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Frequently Asked Questions

Do you use RNase pretreatment prior to RNA isolation from exosomes?

RNase pretreatment is recommended to digest external RNA and ensure you’re profiling only vesicle-enclosed RNA. Perform RNase treatment before lysis to remove free RNA without compromising exosome integrity 

What normalization control should I use for exosomal RNA qPCR?

Standard housekeeping genes aren’t reliable in exosomes. Use synthetic spike-in controls added at extraction (e.g., C. elegans miRNA) or stable small RNAs (like miR-16) for consistent normalization.

Why did I get no RNA after plasma ultracentrifugation of exosomes?

A clear, sticky pellet often means low input or pellet loss. Ensure ≥2 mL plasma, handle pellets gently (avoid over-washing), and check for RNase contamination. If Bioanalyzer shows nothing, verify pellet resuspension and buffer compatibility.

Which marker works when standard housekeeping proteins fail in exosome Westerns?

Cytosolic proteins like actin/GAPDH are often absent. Rely instead on exosome-enriched markers such as CD63, CD9, CD81 or TSG101. If TSG101 is weak, CD63 is a robust alternative.

How do I dissolve a viscous exosome pellet after ultracentrifugation?

Gently resuspend in warm (37 °C) buffer, pipetting slowly. For stubborn pellets, add a small amount of PBS with 0.01 % Tween-20 to ease dispersion—just avoid detergents that interfere with MS.

Do freeze–thaw cycles damage exosomes and affect lipidomics?

Repeated freeze–thaw can disrupt membrane integrity and alter lipid composition. Store aliquots at –80 °C and minimize thaw cycles; consider cryoprotectants like trehalose for better lipid preservation.

How can I prevent aggregation artifacts during NTA?

Filter samples (0.22 µm), dilute appropriately, and avoid excessive freezing/thawing. Adding a carrier protein (e.g., 0.1 mg/mL BSA) helps stabilize vesicles and reduce aggregates.

Does RPMI media itself contain particles detectable by NTA?

Yes, serum-derived vesicles or particulate contaminants in the media can be present. Always run a media‐only blank to identify and subtract background counts.

What are the best practices for exosome flow cytometry setup?

Pre-quantify exosomes (BCA/NTA) to standardize input. Use bead-based capture (anti-CD9/63 beads), stain with fluorescent antibodies, set voltages to detect small events, and include unstained/isotype controls for accurate gating.

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