Exosome Isolation: SiC Purification
Exosome Purification using SiC Chemistry
A bias-free way to capture every RNA, perfect for exosome biomarker discovery.
Norgen’s silicon-carbide (SiC) chemistry binds total RNA without size or GC bias, so you recover the full transcript spectrum, from long mRNAs to fragmented microRNAs, exactly what Exosoome studies need when input is scarce or degraded. In practice, that means cleaner signals, stronger panels, and fewer reruns.
Why use SiC for Exosome projects
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All sizes, no bias. SiC shows uniform affinity across RNA species (including small RNAs), avoiding the size/GC bias typical of silica. Ideal for EV cargo profiling and NGS.
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Higher mapping efficiency for small RNAs. EXTRAClean™ kits report up to a 10× increase in microRNA mapping on sequencing runs—more usable reads, lower cost per sample.
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Downstream-ready RNA. Phenol-free purification delivers RNA that performs in qPCR, small-RNA-seq, and whole-transcriptome workflows.
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Clinical-grade sample coverage. Purpose-built kits for plasma/serum, urine, saliva, and cell culture media let you standardize across cohorts.
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Complete SiC workflows
Standardize from collection & preservation → intact Exosome purification → SiC RNA isolation
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Kits by sample type (quick pick)
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Plasma/Serum: Plasma/Serum Exosome Purification & RNA Isolation (up to 10 mL). The kit underpinning the “up to 10× miRNA mapping” claim.
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Urine: Urine Exosome & Free-Circulating RNA Isolation (up to 30 mL) or Urine Exosome Purification & RNA Isolation (0.25–1 mL). Sequential EV-RNA + cfRNA options.
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Saliva: Saliva Exosome Purification Kit for fresh or preserved saliva.
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Cell Culture Media (CCM): Fast Exosome purification—ideal before SiC RNA extraction; pair with FBS Exosome Depletion kits to clean up media.
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Want to learn more?
Explore the world of RNA with Silicon Carbide Exosome purification
Patented SiC membrane; Exosome capture by design
Norgen Biotek holds CA2929268C covering pH-conditioned binding of extracellular vesicles to silicon carbide with controlled elution or selective depletion. The patent explicitly describes binding and elution of exosomes on an SiC membrane under specific pH conditions. (See also application CA2929268A1.)
Where SiC shines
Low-input cohorts (e.g., ≤1 mL plasma/serum) and variable biobanks with transcript fragmentation.
Discovery + validation that demand full RNA representation (panels, signatures, longitudinal studies).
Frequently Asked Questions
Silica can under-recover small/fragmented RNA; SiC shows minimal size/sequence bias, improving detection of challenging Exosome RNAs.
Yes. Perform Exosome isolation first, then extract Exosome RNA with SiC for comprehensive cargo readouts.
Plasma/serum, urine, saliva, CSF, and cell-culture media are validated across Norgen’s portfolio for exosome work.
No. Norgen’s kits are designed to bind all RNA sizes, including microRNA, without size or GC bias.